Yeast grown in continuous culture systems can detect mutagens with improved sensitivity relative to the Ames test

Use of yeast respiratory adaptation test system to detect chemical mutagens/carcinogens in mammals.

An approach to follow distribution of injected DNA-acting chemicals (mutagens/carcinogens) in animal tissues has been described. This is based on the use of respiratory adaptation (mitochondrial biogenesis) process in Saccharomyces cerevisiae during transition from anaerobic to aerobic state. By virtue of specific interaction of such chemicals with mitochondrial DNA associated with promitochond...

Assay of 855 test chemicals in ten tester strains using a new modification of the Ames test for bacterial mutagens.

Determination of mutagenic activity in bacterial systems has become accepted as an initial step in the evaluation of the carcinogenic potential of new chemicals. In this paper, a bacterial mutagen screening technique is described in which chemicals can be tested in 10 tester strains over a 10,000-fold concentration gradient both with and without metabolic activation. Using this assay, 855 chemi...

Ames, the Ames test, and the causes of cancer.

Improved method for measurement of retinol and didehydroretinol in the modified relative dose response test to detect vitamin A deficiency.

To the Editor: Vitamin A deficiency (VAD) is one of the most devastating dietary deficiencies worldwide. It causes significant increases in childhood and maternal morbidity and mortality among the poor of the nonindustrialized world. Several methods are available to assess VAD. The determination of retinol in blood is one of the most frequently used methods, but it has several disadvantages. Se...

Maize pollen test systems to detect nondisjunction.

Three pollen test systems to detect the induction of nondisjunction in maize are actively being explored. (1) Each member of a tetrad of haploid microspores produced by meiosis contains a single chromosome 6 which carries the only nucleolar organizing region in the maize genome. Thus, each member of a normal tetrad contains one nucleolus. If nondisjunction took place at the first or second meio...